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Abstract: The purpose of the project was to sequence nematode DNA for use in the development of evolutionary (phylogenic) trees for different populations of Caenorhabdis elegans or C. ... The estimated number of nematode species are in the millions, only a few thousand have been named; almost any shovel full of soil, freshwater or sediment is likely to have thousands of worms including the strong possibility of containing new undiscovered species. ... A small fraction of all nematode species are parasites of humans, livestock or agricultural crops. ... elegans, has gained fame and popularity as a research model in almost every nematode related research program. ... Cell migration and many genetically activated effects have been discovered while observing the nematode developmental process. ... elegans out of all the nematode species, is it has been researched by a large number of universities and they have over the years been able to identify five major clades within the phylum. ...
When chousing a target that both organisms have in common is important, for instance u can’t compare the code that produces feathers on a bird with that of a nematode. ...
Materials and Methods:
At the Hubbard Genome Center located on the campus of The University of New Hampshire, the center preformed work towards the goal of completing a nematode phylogenic tree. The process beginning with a plate containing a specific species of nematode is taken and the surface coated with buffer to wash worms to one location off the surface. After a sample of buffer has been placed on a slide nematodes are picked using a wire loop to ensure just the nematode is isolated for the following process.
Nematode cells are separated and burst for the DNA extraction using a -80 freezers or liquid nitrogen. ...
In one case a specific sequence from a species of nematode would not successfully replicate. ... Once all of the species samples had been sequenced using the D2/D3 primer targeting the ribosomal sequence area of the sample nematode genome a phylogenic tree was then created. ... The comparison result showed proof that the data was constant throughout different sequences in each nematode. ...
Discussion:
The results that came from the sequencing of these nematode populations are useful in more ways than one.
Approximate Word count = 2898
Approximate Pages = 11.6
(250 words per page double spaced)
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