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Finding the Unknown
Introduction:
This experiment shows how to find if the unknown contains proteins, carbohydrates, lipids, or if it is inorganic. ... The unknown is a clear liquid, and does not have any odor to it so I theorize that the unknown is water. ... Materials used in determining the identity of the Unknown Substance:
Proteins Carbohydrates Lipids
• millimeter ruler
• Wax pencil
• Wax pencil
• Four clean test tubes
• Biuret Reagent
• Albumin solution
• Pepsin solution
• Starch solution
• water • Test For Starch
• wax pencil
• Five clean test tubes
• Iodine solution
• Starch suspension
• Onion juice
• Potato juice
• water
• Glucose solution
• Heating Pad
• Test For Sugars
• Wax pencil
• Five clean test tubes
• Benedict’s reagent
• Glucose Solution
• Onion Juice
• water
• Potato Juice • Brown paper
• Vegetable oil
• water
The first method in finding the unknown is checking if it contains sugar. Get two clean test tubes and fill one of them to the one centimeter mark with water, and the other tube with the unknown. ... The second method is to see if your unknown contains starch. To check for starch get two clean test tubes fill one up with water to one centimeter, and the second test tube fill it with the unknown to one centimeter. ... Remember for it to contain starch the unknown needs to turn to a yellowish-brown color. Testing if the unknown content has protein is the third method. ... In the second test tube fill the first mark with the unknown, and the second mark with biuret reagent. ... Place a small drop of the unknown on a square of brown paper.
Approximate Word count = 1232 Approximate Pages = 4.9 (250 words per page double spaced)
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