Investigation to find the factors that affect the rateof an enzyme controlled reaction
...hat are joined together to make large ones, the builder enzymes speed these reaction up. An enzyme has an active site, this joins with the substrate, it has a particular shape to fit perfectly with the substrate. To make sure that my experiment is a fair one I will do the following processors; · I will not add any substances to change the PH · Keep the temperature the same but this may be changed due to the room temperature. For this investigation I will need the following equipment; · Spotting tile · Iodine · Amylase · Starch · Test tubes · Boiling tubes · Pipette · Stop clock For this investigation I predict that when the concentration increases the rate of reaction increases. I believe this because when you add more concentration the rate of reaction increases because the molecules are constantly moving and bumping into each other. A substance molecule collides with the right enzyme molecule. It fits into a depression on the surface on the enzyme called the active site. The molecules of the product leave the active site, freeing it for another substrate molecule. Receive all of the apparatus from the list above. Put one drop of iodine into each well of the spotting tile using the pipette. Get a test tube and add 5cm cubed of starch solution and then 1cm cubed of amylase and start the stop clock immediately. Every 30 seconds take a drop of the solution and add it to a well on the spotting tile repeat this every 30 seconds until the solution has gone back to its original iodine colour, when this happens stop the clock and record your time. You need to repeat this but the next time with 2,3,4,5 drops of the solution with the iodine at the beginning and then add a drop every 30 seconds of the solution. Now I have done this investigation I can now produce my table of results; Drops Repeat 1 2 3 4 5 1 3.30 min 4.30 min 2 min 2 min 1.30 min 2 4 min 4 min 2.30 min 2 min 2 min 3 4 min 3.30 min 2.30 min 2 min 2 min Average 3.50 min 3.40 mi...