Electrophoretic Analysis of LDH in Cows, Goats, Horses, and Sheep
...ttor we inserted 15 µl of each type of serum into the sample wells. After filling the wells, the lid was placed on the unit and the power supply was switched on. When the colored bands reached the point of being about two to three centimeters away from the end of the gel, the power was turned off and the lid was removed. We then put the gel into a staining tray and added 20 mL of stain. After waiting for 30 minutes the gels were removed and analyzed. Cow, goat, and sheep will have more similar electrophoretic patterns for LDH than that of the horse. In this lab we were comparing the properties of lactate dehyrogenase, a protein, in different ungulate mammals. With this, we will compare cows, goats, horses, and sheep to find out which are most closely related. The isoenzymes that each of the animals produce will be the factor used to compare them. We first took our lactate dehyrogenase gel and placed it on the bed of the electrophoresis unit, which will act as our power source, and then we poured a tris-glycine buffer into the unit making sure that all of the wells have been filled. Then using a pipe...