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... Edexcel AS/Advanced GCE Biology and Human Biology Coursework Guide 133
Unit 6 Advanced GCE
T2 Exemplar Coursework
Study 3
Comparing the activity of yeast in different sugars.
134 UA007544 −ハEdexcel AS/Advanced GCE Biology and Human Biology Coursework Guide
3
Abstract
This study looks at the cellular respiration of yeast using mono and disaccharide sugars. ... Edexcel AS/Advanced GCE Biology and Human Biology Coursework Guide 135
INTRODUCTION
Yeast is a unicellular fungus and the most common form used in brewing is Saccharomyces
sp. ...
The disaccharides used are made up of the following monomers;
MALTOSE = GLUCOSE + GLUCOSE
LACTOSE = GALACTOSE + GLUCOSE
SUCROSE = GLUCOSE + FRUCTOSE
The individual structures are as follows ;
136 UA007544 −ハEdexcel AS/Advanced GCE Biology and Human Biology Coursework Guide
I would expect glucose to give the highest rate of respiration as it is directly
phosphorylated in the first stages of glycolysis. ... However to make more
accurate predictions it would be necessary to study the main 3-D structures of each
form of each sugar in detail. This can be complex since some such as fructose exist in
more than one basic form (a 6-sided ring and a 5-sided ring). ... Edexcel AS/Advanced GCE Biology and Human Biology Coursework Guide 137
PLAN
Basic hypothesis Glucose and Maltose will produce the highest rates of respiration in
yeast compared with sucrose, fructose, galactose and lactose. ...
138 UA007544 −ハEdexcel AS/Advanced GCE Biology and Human Biology Coursework Guide
Control of variables
Volume of substrate to be constant at 5cm3
Concentration of substrate kept constant at 2%
Volume of yeast to be kept constant at 20cm3
Concentration and type of yeast to be kept constant by using the same culture and
stirring to ensure even dispersion before extracting each sample. ... Edexcel AS/Advanced GCE Biology and Human Biology Coursework Guide 139
Results
Volume of carbon dioxide produced using glucose
Time(mins) 2 4 6 8 10
Vol cm3 (a) 1 1.
Approximate Word count = 1526
Approximate Pages = 6.1
(250 words per page double spaced)
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