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Beetroot Experiment.
Aim
The aim of this practical is to use beetroot to examine the effect of temperature on cell membranes and relate the effects observed to membrane structure.
Introduction
The beetroot pigment characteristic is red/purple colour, which is found in the vacuole of the cells. If a cell is damaged in a beetroot plant and the membrane is broken, the anthocyanin bleeds from the cells like a dye. ...
Because we are experimenting with the effects of temperature on the membrane, we will place the samples of beetroot into a water baths of varying temperatures and measure the colour change in the water. ... The dependant variable (DV) in this experiment is colour change in water caused by anthocyanin leakage, which is measured using % transmission. ...
Method
Cut sections from a single beetroot using a size 4 cork borer. ...
Wash the slices with distilled water and blot dry until all the beetroot pigment has stopped leaking through from the cells that were damaged, when using the cork borer. ... Place one of the beetroot sections into each of the test tubes
Leave for 30 minutes in the water baths.
Remove beetroot sections; shake the water/solution to disperse the dye. ...
Results
Class results of the experiment
% Transmission
0 °C 10 °C 20 °C 30 °C 40 °C 50 °C 60 °C 70 °C
88 97 92 71 83 72 16 15
92 93 80 70
78 89 81 57 12 11
91 98 97 87 64 66 16 17
84 79 69 54 15 12
86 80 80 65 50 11 13
90 85 80 87 64 64 21 11
88 91 91 74 67 16 10
* the blank spaces indicate anomalous results.
Approximate Word count = 1378
Approximate Pages = 5.5
(250 words per page double spaced)
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