investigation to determine theeffect of pH on the rate of an enzyme catalysed reaction using the
... ENZYME THEORY 4. ... I will be using pH 4. ... 0 I will then put 5cm of pH 4. ... Then I will do this process two more times to get an average for that pH. ... Then I will repeat this whole process another five times at pH 5. ... Always using three test tubes at each pH. ... Apparatus · Stopwatch · Electric balance · Ruler calibrated in mm · Potato core cutter · Boiling tubes · Delivery tubes · Test tubes · Beakers · 2 Syringes · Hydrogen peroxide · pH Buffers 4-9 · Water · Heating apparatus · Test tube holder · Scalpel Prediction In a previous experiment with pH 5. ... 0, there was a gradual rise in activity from pH 5. ... 0 & pH 8. ... At pH 5.0, I counted 60 bubbles at pH 7.0, I counted 71 bubbles and at pH 9. ... In this experiment I will be using pH buffers to control the pH level. I predict that the enzyme at ph 4, will produce 55 bubbles in one minute. I predict that at pH 5, the reaction between the enzyme catalase and hydrogen peroxide will produce 60 bubbles in one minute. I predict that at pH 6 the potato chip will produce 65 bubbles in one minute. I predict that at pH 7 the potato chip will produce 70 bubbles in one minute. I predict that at pH 8 the potato chip will produce 65 bubbles in one minute. I predict that at pH 9 the potato chip will produce 60 bubbles in one minute. ... Obtaining Evidence Table to show the number of bubbles counted in one minute at different pHs PH NUMBER OF BUBBLES 1ST 2ND 3RD TRIAL 4. ... PH NUMBER OF BUBBLES. ... PH 4 PH5 PH6 PH7 PH8 PH9 WEIGHT 1. ... 00g PH NUMBER OF BUBBLES 1ST 2ND 3RD Average 4. ... 0 51 57 55 54 Enzyme Theory In this experiment I am testing the effect of ph on the enzyme catalase. Enzymes are biological catalysts, which alter the rate of a reaction without being changed themselves. ... The chemical reaction accelerated by catalase is written: ENZYME HYDROGEN PEROXIDE WATER +OXYGEN CATALASE Acid or alkaline conditions alter the chemical properties of proteins, including enzymes. Most enzymes work best at a particular level of acidity or alkalinity (ph). The protein-digesting enzyme in your stomach, for example, works well at an acidity of ph 2.