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... The gram-positive bacteria retain the purple color from the crystal violet and the gram-negative bacteria retain the red color from the safranin. ... aureus to appear about the same because Gram positive cells will incorporate little or no counter stain and will remain blue-violet in appearance. ... coli to take on the blue color from the crystal violet because it would not have been washed out by the decolouriser and therefore would not have the pink color that usually makes them easily distinguishable from the Gram positives. ... Gram positive cell walls are about five times as rich in peptidoglycan as gram-negative cell walls. In addition, gram-negatives have a second membrane (chemically different from the plasma membrane) external to the cell wall. ... Gram staining is the most practical and efficient way to classify and unknown bacterium. ... It is a gram + cocci and one disease it causes food poisoning. ...
Streptococcus pneumoniae is another gram + cocci that can cause many diseases such as pneumonia, meningitis and otitis media. ...
Salmonella enterica is a gram – bacteria which also cause a variety of diseases. ...
Pseudomonas aeroginosa is a gram - bacillus that is found in soil and water. ...
The Importance and Practical Applications behind Gram Staining:
About one third of all patients admitted to general hospitals have, or develop, infections, that are caused by bacteria and fungi. Usually the organisms cannot be identified by clinical presentation alone and should be examined in the form of a Gram-stained smear. Gram stain of collected specimens may help:
1. Determine the adequacy of the specimen for culture
The Gram-stained smear is useful in judging the adequacy of the specimen obtained. ... Make a presumptive etiologic diagnosis and early clinical decisions
Immediate examination of a Gram-stained smear of material from the infection site can often provide important data on which to base early clinical decisions, prior to the availability of culture results.
Approximate Word count = 1557
Approximate Pages = 6.2
(250 words per page double spaced)
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