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Introduction: In this experiment, I mixed plasmid DNA containing the gene for green fluorescent protein with E. coli bacteria to discover how bacterial cells can be genetically transformed with plasmid DNA containing a jellyfish gene. Plasmids occur naturally in many bacteria. Plasmid DNA is replicated and expressed inside bacterial cells. Copies of plasmid can also move from one bacterial cell to another. Biologists can “cut and paste” certain genes into a plasmid through restriction enzymes. In this lab, the plasmid that contains different genes cut out through restriction enzymes is called pGLO. This plasmid contains genes including one from a bioluminescent jellyfish. In the lab, copies of the pGLO plasmid will be moved into bacterial cells through the process of genetic transformation. With this information, I can conclude that bacterial cells can be genetically transformed with plasmid DNA containing a jellyfish gene because the jellyfish gene was “cut out and pasted” onto the plasmid using restriction enzymes then the plasmid was inserted into the bacterial cell which now gives the bacterial cell the certain bioluminescent gene from the jellyfish allowing it to glow. *Information came from pGLO lab Materials and Methods: Before starting the transformation procedure we observed the starter plate with colonies of E. coli. Then we shined the UV lamp on the colonies and observed the results. Then we shined the UV lamp on the tube of the pGLO plasmid DNA to determine whether the DNA glows. Then we recorded our observations. Then we labeled one of the closed microcentrifuge tubes with a plus sign (for “with plasmid” and the other with a minus sign (“for without plasmid”). Then we put both the tubes in the foam tube rack.
Approximate Word count = 1122
Approximate Pages = 4.5
(250 words per page double spaced)
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